Individual Subject Meta-Analysis of Parameters forGiardia duodenalisShedding in Animal Experimental Models

[摘要] Giardia duodenalisis a zoonotic protozoan parasite with public health importance worldwide. While articles about animal model infectivity have been published forG. duodenalis, the studies have used diverse protocols and parameters to evaluate the infectivity of this protozoan parasite. Hence, the objectives of this study were to (1) conduct a meta-analysis of published literature for cyst shedding and diarrhea outcomes in animal models and (2) develop recommendations to help standardize experimental dose response studies. Results showed that, for the outcome of cyst shedding in faeces, the covariates of infective stage (cyst versus trophozoite),Giardiadose, and the interactions between doses and infective stage, as well as dose and species of experimental host, were all significant (Pvalue ≤ 0.05). This study suggests inoculation of the experimental host with cysts rather than trophozoites and administration of higher doses ofGiardiawill most likely result in cyst shedding. Based on the results of this meta-analysis, the infective stage (cyst versus trophozoite), parasite dose, and the interactions between dose and infective stage, as well as dose and species of experimental host, should be considered when designing experimental dose response studies that will assist in the study of zoonotic neglected tropical diseases globally.

[学科分类] 基础医学