Detection of the Epstein-Barr Virus and DNA-Topoisomerase II-αin Recurrent and Nonrecurrent Giant Cell Lesion of the Jawbones

[摘要] The aims of this study were to determine whether the expression of Topo II-αcorrelates with presence ofEBV in giant cell lesion of the jawbones and whether it is predictive of clinical biologic behavior of these lesions. Paraffin-embedded tissues from 8 recurrent and 7 nonrecurrent cases of bony GCLs and 9 peripheral giant cell lesions (PGCLs) as a control group were assessed for the expression of EBV and Topo II-αusing immunohistochemistry. The results showed positive staining for Topo II-αin mononuclear stromal cells (MSCs) and multinucleated giant cells (MGCs). Studentt-test showed that mean Topo II-αlabelling index (LI) in recurrent cases was significantly higher than that in non-recurrent cases (P=0.0001). Moreover, Spearman's correlation coefficients method showed a significant correlation between DNA Topo II-αLI and both of gender and site in these lesions. Moderate EBV expression in relation to the highest Topo II-αLI was observed in two cases of GCT. It was concluded that high Topo II-αLIs could be identified as reliable predicators for the clinical behavior of GCLs. Moreover, EBV has no etiological role in the benign CGCLs in contrast to its role in the pathogenesis of GCTs.

[学科分类] 基础医学