Genetic Variation betweenBiomphalaria alexandrinaSnails Susceptible and Resistant toSchistosoma mansoniInfection

[摘要] Much effort has been made to control schistosomiasis infection in Egypt. However, enduring effects from such strategies have not yet been achieved. In this study, we sought to determine the genetic variability related to the interaction betweenBiomphalaria alexandrinasnails andSchistosoma mansoni. Using RAPD-PCR with eight (10 mers) random primers, we were able to determine the polymorphic markers that differed between snails susceptible and resistant toSchistosoma mansoniinfection using five primers out of the eight. Our results suggest that the RAPD-PCR technique is an efficient means by which to compare genomes and to detect genetic variations between schistosomiasis intermediate hosts. The RAPD technique with the above-noted primers can identify genomic markers that are specifically related to theBiomphalaria alexandrina/Schistosoma mansonirelationship in the absence of specific nucleotide sequence information. This approach could be used in epidemiologic surveys to investigate genetic diversity amongBiomphalaria alexandrinasnails. The ability to determine resistant markers inBiomphalaria alexandrinasnails could potentially lead to further studies that use refractory snails as agents to control the spread of schistosomiasis.

[学科分类] 基础医学