In SilicoPrediction andIn VitroCharacterization of Multifunctional Human RNase3

[摘要] Human ribonucleases A (hRNaseA) superfamily consists of thirteen members with high-structure similarities but exhibits divergent physiological functions other than RNase activity. Evolution of hRNaseA superfamily has gained novel functions which may be preserved in a unique region or domain to account for additional molecular interactions. hRNase3 has multiple functions including ribonucleolytic, heparan sulfate (HS) binding, cellular binding, endocytic, lipid destabilization, cytotoxic, and antimicrobial activities. In this study, three putative multifunctional regions,34RWRCK38(HBR1),75RSRFR79(HBR2), and101RPGRR105(HBR3), of hRNase3 have been identified employingin silicosequence analysis and validated employingin vitroactivity assays. A heparin binding peptide containing HBR1 is characterized to act as a key element associated with HS binding, cellular binding, and lipid binding activities. In this study, we provide novel insights to identify functional regions of hRNase3 that may have implications for all hRNaseA superfamily members.

[学科分类] 基础医学