HPTLC Analysis of Bioactivity Guided Anticancer Enriched Fraction of Hydroalcoholic Extract ofPicrorhiza kurroa

[摘要] Objective. Hydroalcoholic extract ofPicrorhiza kurroaand its fractions were subjected toin vitroscreening for cytotoxicity; further best active fraction (BAF) obtained was tested against Ehrlich ascites carcinoma (EAC) model in Balb/c mice after its quality control analysis.Methods. Cytotoxicities of all the fractions and mother extract ofP. kurroawere determined, using MTT assay on breast cancer (MCF-7, MDA-MB 231) and cervical cancer (HeLa, SiHa) cell lines. Metabolic fingerprinting was developed using HPTLC with quantification of biomarkers (cucurbitacins B and E; betulinic acid; picrosides 1 and 2; and apocynin) in BAF. The EAC tumor-bearing mice were used forin vivoanticancer activity after oral administration (50 mg Kg−1) for 10 days.Results. Cytotoxicity assay of mother extract and its fractions over breast cancer and cervix cancer cell lines showed that dichloromethane (DCM) fraction was most cytotoxic (IC5036.0–51.0 µg mL−1at 72 h). Oral administration of DCM fraction showed significant reduction in tumor regression parameters, viable tumor cell count and restoration of hematological parameters may be due to presence of cucurbitacins B and E; betulinic acid; picrosides 1 and 2; and apocynin, as compared to the untreated mice of the control group.Conclusion. The DCM fraction ofP. kurroadisplayed potent anticancer activity and can be further explored for the development of a potential candidate for cancer therapy.

[学科分类] 基础医学