Prevalence ofHelicobacter pylori vacA,cagA, andiceAGenotypes in Cuban Patients with Upper Gastrointestinal Diseases

[摘要] Virulence factors ofHelicobacter pylorican predict the development of different gastroduodenal diseases. There are scarce reports in Cuba aboutH. pyloriisolates genotyping. The aim of the present investigation was to identify allelic variation of the virulence genesvacA,cagA, andiceAin sixty-eight patients diagnosed asH. pyloripositive by culture. In seven out of 68 patients, strains from both gastric regions were obtained and considered independent. DNA was extracted from all theH. pyloristrains and evaluated by PCR-genotyping. ThevacAs1 allele,cagAgene, andiceA2allele were the most prevalent (72.0%, 56.0%, and 57.3%, respectively). Alleles from m-region showed a similar frequency as s1a and s1b subtypes. The presence of multipleH. pylorigenotypes in a single biopsy and two gastric region specimens were found. Significant statistical association was observed betweeniceA2allele and patients with non-peptic ulcer dyspepsia (NUD) (P=0.037) as well as virulence genotypes (s1,s1m2) and patients over 40 years old (P<0.05). In conclusion, the results demonstrated a high prevalence ofH. pylorivirulent genotypes in Cuban patients over 40 years old whileiceA2alleles demonstrated a good specificity in patients with NUD.

[学科分类] 基础医学