Expression of Smooth Muscle Calponin in Synovial Sarcoma

[摘要] Purpose.Histogenesis of synovial sarcoma remains controversialand reliable molecular markers for diagnosis are necessary. Expression of basic calponin,a smooth muscle differentiation-specific actin-binding protein, was studied insynovial sarcoma.Subjects and Methods.The basic calponin gene and the geneproduct were analyzed by reverse transcription PCR analysis (RT-PCR) andimmunohistochemistry in 14 synovial sarcomas and a human synovial sarcomacell line (HS-SY-II).Results and Discussion.Immunoreactivity for basic calponinwas detected in the cytoplasm of 6 synovial sarcomas (43% positive). In the basiccalponin-positive tumors and the HS-SY-II cells, expression for smooth muscle-specificgenes, including basic calponin and SM22α , was detected by RT-PCR, suggesting alineage relationship between synovial sarcoma cells and smooth muscle-likemesenchymal cells.Conclusions.A subset of synovial sarcomas expressing the basiccalponin gene and the gene product were identified. The basic calponin may havepotential utility as a novel molecular marker identifying certain synovial sarcomas.

[学科分类] 肿瘤学