Protein and Chemical Microarrays—Powerful Tools for Proteomics

[摘要] In the last few years, protein and chemical microarrays haveemerged as two important tools in the field of proteomics.Specific proteins, antibodies, small molecule compounds,peptides, and carbohydrates can now be immobilized on solidsurfaces to form high-density microarrays.Depending on theirchemical nature, immobilization of these molecules on solidsupport is accomplished by in situ synthesis, nonspecificadsorption, specific binding, nonspecific chemical ligation, orchemoselective ligation.These arrays of molecules can then beprobed with complexanalytessuch as serum, total cell extracts,and whole blood. Interactions between the analytes and theimmobilized array of molecules are evaluated with a number ofdifferent detection systems.In this paper, variouscomponents, methods, and applications of the protein and chemicalmicroarray systems are reviewed.

[学科分类] 基础医学