Differential Behavior Between Isolated and Aggregated Rabbit Auricular Chondrocytes on Plastic Surfaces

[摘要] A knowledge of the behavior of chondrocytes in culture isrelevant for tissue engineering. Chondrocytes dedifferentiate toa fibroblast-like phenotype on plastic surfaces.Dedifferentiation is reversible if these cells are then culturedin suspension. In this report a description is given of how whenchondrocyte aggregates formed in suspension are next seeded onplastic, most of them attach as round or polygonal cells. Thismorphological differentiation, with synthesis of type IIcollagen, is stable for long culture periods. This simple methodcan be of use as a model for studies of chondrocyte behavior onplastic. The results indicate that in addition to cultureconditions, such as cell isolation method or cell density,chondrocyte behavior on plastic depends on the presence ofaggregates.

[学科分类] 基础医学