Adhesion to Vitronectin and Collagen I Promotes Osteogenic Differentiation of Human Mesenchymal Stem Cells

[摘要] The mechanisms controlling human mesenchymal stemcells (hMSC) differentiation are not entirely understood. Wehypothesized that the contact with extracellular matrix (ECM)proteins normally found in bone marrow would promote osteogenicdifferentiation of hMSC in vitro. To test this hypothesis, wecultured hMSC on purified ECM proteins in the presence or absenceof soluble osteogenic supplements, and assayed for the presence ofwell-established differentiation markers (production ofmineralized matrix, osteopontin, osteocalcin, collagen I, andalkaline phosphatase expression) over a 16-day time course. Wefound that hMSC adhere to ECM proteins with varying affinity(fibronectin > collagen I ≥ collagenIV ≥ vitronectin > laminin-1) and through distinct integrin receptors.Importantly, the greatest osteogenic differentiation occurred incells plated on vitronectin and collagen I and almost nodifferentiation took place on fibronectin or uncoated plates. Weconclude that the contact with vitronectin and collagen I promotesthe osteogenic differentiation of hMSC, and that ECM contactalone may be sufficient to induce differentiation in these cells.

[学科分类] 基础医学