Toward Engineering Synthetic Microbial Metabolism

[摘要] The generation of well-characterized parts and the formulation of biological design principles in synthetic biology are laying the foundation for more complex and advanced microbial metabolic engineering.Improvements inde novoDNA synthesis and codon-optimization alone are already contributing to the manufacturing of pathway enzymes with improved or novel function.Further development of analytical and computer-aided design tools should accelerate the forward engineering of precisely regulated synthetic pathways by providing a standard framework for the predictable design of biological systems from well-characterized parts.In this review we discuss the current state of synthetic biology within a four-stage framework (design, modeling, synthesis, analysis) and highlight areas requiring further advancement to facilitate true engineering of synthetic microbial metabolism.

[学科分类] 基础医学