The Fur Transcription Regulator and Fur-Regulated Genes inClostridium botulinumA ATCC 3502

[摘要] Clostridium botulinumis a spore-forming bacterium that can produce a very powerful neurotoxin that causes botulism. In this study, we have investigated the Fur transcription regulators inClostridium botulinumand Fur-regulated genes inClostridium botulinumA ATCC 3502. We found that gene loss may be the main cause leading to the different numbers of Fur transcription regulators in differentClostridium botulinumstrains. Meanwhile, 46 operons were found to be regulated by the Fur transcription regulator inClostridium botulinumA ATCC 3502, involved in several functional classifications, including iron acquisition, iron utilization, iron transport, and transcription regulator. Under an iron-restricted medium, we experimentally found that a Fur transcription regulator (CBO1372) and two operons (DedA, CBO2610–CBO2614 and ABC transporter, CBO0845–CBO0847) are shown to be differentially expressed inClostridium botulinumA ATCC 3502. This study has provided-us novel insights into the diversity of Fur transcription regulators in differentClostridium botulinumstrains and diversity of Fur-targeted genes, as well as a better understanding of the dynamic changes in iron restriction occurring in response to this stress.

[学科分类] 基础医学