Distribution of Myosin Attachment Times Predicted from Viscoelastic Mechanics of Striated Muscle

[摘要] We demonstrate that viscoelastic mechanics of striated muscle, measured as elastic and viscous moduli, emerge directly from the myosin crossbridge attachment time,tatt, also called time-on. The distribution oftattwas modeled using a gamma distribution with shape parameter,p, and scale parameter,β. At 5 mM MgATP,βwas similar between mouseα-MyHC (16.0±3.7 ms) andβ-MyHC (17.9±2.0 ms), andpwas higher (P<0.05) forβ-MyHC (5.6±0.4no units) compared toα-MyHC (3.2±0.9). At 1 mM MgATP,papproached a value of 10 in both isoforms, butβrose only in theβ-MyHC (34.8±5.8 ms). The estimated meantatt(i.e.,pβproduct) was longer in theβ-MyHC compared toα-MyHC, and became prolonged in both isoforms as MgATP was reduced as expected. The application of our viscoelastic model to these isoforms and varying MgATP conditions suggest thattattis better modeled as a gamma distribution due to its representing multiple temporal events occurring withintattcompared to a single exponential distribution which assumes only one temporal event withintatt.

[学科分类] 基础医学