Collagen-Based Films Containing Liposome-Loaded Usnic Acid as Dressing for Dermal Burn Healing

[摘要] The aim of this study was assess the effect of collagen-basedfilms containing usnic acid as a wound dressing for dermal burnhealing. Second-degree burn wounds were performed in forty-fiveWistar rats, assigned into nine groups: COL—animals treatedwith collagen-based films; PHO—animals treated with collagenfilms containing empty liposomes; UAL—animals treated withcollagen-based films containing usnic acid incorporated intoliposomes. After 7, 14, and 21 days the animals were euthanized. On 7th day there was a moderate infiltration of neutrophils, inUAL, distributed throughout the burn wounds, whereas in COL andPHO, the severity of the reaction was slighter and still limitedto the margins of the burn wounds. On the 14th day, theinflammatory reaction was less intense in UAL, with remarkableplasma cells infiltration. On the 21st day, there was reduction ofthe inflammation, which was predominantly composed of plasma cellsin all groups, particularly in UAL. The use of the usnic acidprovided more rapid substitution of type-III for type-I collagenon the 14th day, and improved the collagenization density on the21st day. It was concluded that the use of reconstituted bovinetype-I collagen-based films containing usnic acid improved burnhealing process in rats.

[学科分类] 基础医学