Molecular Characterization of theGhrelinandGhrelin ReceptorGenes and Effects on Fat Deposition in Chicken and Duck

[摘要] Ghrelin (GHRL) and its receptor (GHSR) are involved in various bioactivities. In this study, the complete cDNA and5′flanking region of the duckGHRL(dGHRL) gene and a 3717 bp fragment of the duckGHSR(dGHSR) gene were obtained. A total of 19, 8, 43, and 48 SNPs identified in 2751, 1358, 3671, and 3567 bp of the chickenGHRL(cGHRL), chickenGHSR(cGHSR),dGHRL, anddGHSRgenes, respectively. BothcGHRLanddGHRLwere expressed predominantly in the proventriculus, whereas the highest mRNA levels ofcGHSRanddGHSRwere detected in the breast muscle and pituitary. Association analysis showed that C-2047G, A-2355C, and A-2220C of thecGHRLgene were significantly associated with abdominal fat weight (AFW;P=.01), crude protein content of leg muscle (CPCLM;P=.02), and CPCLM (P=.0009), respectively. C-1459T of the cGHSR gene was also significantly associated with CPCLM (P=.0004). C-729T of dGHRL andA3427T of dGHSR were both significantly associated with subcutaneous fat thickness (SFT;P=.04). It was indicated by this study that theGHRLandGHSRgenes were related to fat deposition in both chicken and duck.

[学科分类] 基础医学