An Improved Method to Knock Out theasdGene ofSalmonella entericaSerovar Pullorum

[摘要] Anasd-deleted (Δasd) mutant ofSalmonella entericaserovar Pullorum (SP) was constructed using an improved method of gene knockout by combining theπ-suicide plasmid system with the Red Disruption system. Theasdgene was efficiently knocked out by the recombinant suicide vector, which replaced theasdgene with theCmRgene. Based on the balanced lethal host-vector system, the phenotype of theΔasdmutant was further defined. The improved method was simpler and more effective than previously reported conventional methods.

[学科分类] 基础医学