Biosynthesis of Antitumoral and Bactericidal Sanguinarine

[摘要] A simple, rapid, and reliable TLC method for the separation and determinationof sanguinarine has been established. This intensively studied biologically active alkaloid has a wide range ofpotentially useful medicinal properties, such as antimicrobial, antiinflammatory, and antitumoral activities. Sanguinarine has also been incorporated intoexpectorant mixtures and has a strong bactericidaleffect upongram-positive bacteria, particularlyBacillus anthracisandstaphylococci. These medicinal properties are due to theinteraction of sanguinarine with DNA. A fibre-optic-basedfluorescence instrument for in situ scanning was used forquantitative measurements. The sanguinarine was determined overthe range 5–40 ng and a detection limit of 1.60 ng. Themethod was applied to the quantification of sanguinarine in tissueculture extracts ofChelidonium majus L.

[学科分类] 基础医学