Investigation of Microencapsulated BSH ActiveLactobacillusin the Simulated Human GI Tract

[摘要] This study investigated the use of microencapsulated bile salt hydrolase (BSH) overproducingLactobacillus plantarum80 cells for oral delivery applications using a dynamic computer-controlled model simulating the human gastrointestinal (GI) tract. Bile salt deconjugation rates for microencapsulated BSH overproducing cells were 4.87±0.28 μmol/g microcapsule/h towards glycoconjugates and 0.79±0.15 μmol/g microcapsule/h towards tauroconjugates in the simulated intestine, a significant (P<.05) increase over microencapsulated wild-type cells. Microcapsules protected the encased cells in the simulated stomach prior to intestinal release, maintaining cell viability above109 cfu/mL at pH 2.5 and 3.0 and above106 cfu/mL at pH 2.0 after 2-hour residence times. In the simulated intestine, encased cell viability was maintained above1010 cfu/mL after 3, 6, and 12-hour residence times in bile concentrations up to 1.0%. Results show that microencapsulation has potential in the oral delivery of live BSH active bacterial cells. However,in vivotesting is required.Erratum of “Investigation of Microencapsulated BSH ActiveLactobacillusin the Simulated Human GI Tract”dx.doi.org/10.1155/2009/730476

[学科分类] 基础医学