Distinction between Pore Assembly by Staphylococcalα-Toxin versus Leukotoxins

[摘要] The staphylococcal bipartite leukotoxins and the homoheptamericα-toxin belong to the same family ofβ-barrel pore-forming toxins despite slight differences. In theα-toxin pore, the N-terminal extremity of each protomer interacts as a deployed latch with two consecutive protomers in the vicinity of the pore lumen. N-terminal extremities of leukotoxins as seen in their three-dimensional structures are heterogeneous in length and take part in theβ-sandwich core of soluble monomers. Hence, the interaction of these N-terminal extremities within structures of adjacent monomers is questionable. We show here that modifications of their N-termini by two different processes, using fusion with glutathione S-transferase (GST) and bridging of the N-terminal extremity to the adjacentβ-sheet via disulphide bridges, are not deleterious for biological activity. Therefore, bipartite leukotoxins do not need a large extension of their N-terminal extremities to form functional pores, thus illustrating a microheterogeneity of the structural organizations between bipartite leukotoxins andα-toxin.

[学科分类] 基础医学