ImagingGABAcReceptors with Ligand-ConjugatedQuantum Dots

[摘要] We report a methodology for labeling theGABAcreceptor on the surface membrane of intact cells. This work builds upon our earlier work with serotonin-conjugated quantum dots and our studies with PEGylated quantum dots to reduce nonspecific binding. In the current approach, a PEGylated derivative of muscimol was synthesized and attached via an amide linkage to quantum dots coated in an amphiphilic polymer derivative of a modified polyacrylamide. These conjugates were used to imageGABACreceptors heterologously expressed in Xenopus laevisoocytes.

[学科分类] 基础医学