Interaction ofHelicobacter pyloriwith C-Type Lectin Dendritic Cell-Specific ICAM Grabbing Nonintegrin

[摘要] In this study we asked whetherHelicobacter pyloriwhole cells and lipopolysaccharide (LPS) utilize sugar moieties of Lewis (Le) antigenic determinants to interact with DC-SIGN (dendritic cell specific ICAM grabbing nonintegrin) receptor on dendritic cells (DCs). For this purpose the soluble DC-SIGN/Fc adhesion assay and the THP-1 leukemia cells with induced expression of DC-SIGN were used. We showed that the binding specificity of DC-SIGN withH. pyloriLeX/Ypositive whole cells andH. pyloriLPS of LeX/Ytype was fucose dependent, whereas in LeXYnegativeH. pyloristrains and LPS preparations without Lewis determinants, this binding was galactose dependent. The binding of soluble synthetic LeXand LeYto the DC-SIGN-like receptor on THP-1 cells was also observed. In conclusion, the LeXYdependent as well as independent binding ofH. pyloriwhole cells andH. pyloriLPS to DC-SIGN was described. Moreover, we demonstrated that THP-1 cells may serve as anin vitromodel for the assessment ofH. pylori-DC-SIGN interactions mediated by LeXand LeYdeterminants.

[学科分类] 基础医学