cDNA-SRAP and Its Application in Differential Gene Expression Analysis: A Case Study inErianthus arundinaceum

[摘要] Erianthus arundinaceumis a wild relative species of sugarcane. The aim of this research was to demonstrate the feasibility of cDNA-SRAP for differential gene expression and to explore the molecular mechanism of drought resistance inE. arundinaceum. cDNA-SRAP technique, for the first time, was applied in the analysis of differential gene expression inE. arundinaceumunder drought stress. In total, eight differentially expressed genes with length of 185–427 bp were successfully isolated (GenBank Accession numbers: EU071770, EU071772, EU071774, EU071776, EU071777, EU071779, EU071780, and EU071781). Based on their homologies with genes in GenBank, these genes were assumed to encode ribonuclease III, vacuolar protein, ethylene insensitive protein, aerobactin biosynthesis protein, photosystem II protein, glucose transporter, leucine-rich repeat protein, and ammonia monooxygenase. Real-time PCR analysis on the expression profiling of gene (EU071774) encoding ethylene-insensitive protein and gene (EU071781) encoding ammonia monooxygenase revealed that the expression of these two genes was upregulated both by PEG and ABA treatments, suggesting that they may involve in the drought resistance ofE. arundinaceum. This study constitutes the first report of genes activated inE. arundinaceumby drought stress and opens up the application of cDNA-SRAP in differential gene expression analysis inE. arundinaceumunder certain stress conditions.

[学科分类] 基础医学