Comparative Genomics of Ten Solanaceous Plastomes

[摘要] Availability of complete plastid genomes of ten solanaceous species,Atropa belladonna,Capsicum annuum,Datura stramonium,Nicotiana sylvestris,Nicotiana tabacum,Nicotiana tomentosiformis,Nicotiana undulata,Solanum bulbocastanum,Solanum lycopersicum, andSolanum tuberosumprovided us with an opportunity to conduct theirin silicocomparative analysis in depth. The size of complete chloroplast genomes and LSC and SSC regions of three species ofSolanumis comparatively smaller than that of any other species studied till date (exception: SSC region ofA. belladonna). AT content of coding regions was found to be less than noncoding regions. A duplicate copy of trnH gene inC. annuumand two alternative tRNA genes for proline inD. stramoniumwere observed for the first time in this analysis. Further, homology search revealed the presence of rps19 pseudogene and infA genes inA. belladonnaandD. stramonium, a region identical to rps19 pseudogene inC. annumand orthologues of sprA gene in another six species. Among the eighteen intron-containing genes, 3 genes have two introns and 15 genes have one intron. The longest insertion was found in accD gene inC. annuum. Phylogenetic analysis using concatenated protein coding sequences gave two clades, one forNicotianaspecies and another forSolanum,Capsicum,Atropa, andDatura.

[学科分类] 生物技术