Performance of a reflectometric techniquefor serum alanine aminotransferasedeterminations

[摘要] The Ames Seralyzer uses solid phase chemistry coupled withreflectance spectroscopy to measure levels of serum alanineaminotransferase (serum ALT). The study reported here wascarried out to evaluate the performance of the Seralyzer methodcompared to conventional analyses carried out on a Coulter Dacosdiscrete random access analyser using Boehringer (BCL) reagentsconforming to SCE specications at 37° C. Clinically acceptableaccuracy and precision were obtained. The effect of bilirubin andhaemoglobin on the test was investigated. Increased levels ofbilirubin in sera did not affect the estimation but the presence ofhigh haemoglobin levels produced significant interference. Theoverall practicality of the Seralyzer was assessed and the methodwas found to be particularly suitable for the estimation of urgent orhigh risk samples in the laboratory or for use by suitably trainedpersonnel in other clinical areas.

[学科分类] 分析化学