Automation of environmental ELISAs

[摘要] ELISAs for pesticides and herbicides in environmental andagricultural samples are becoming very important in screeningapplications [1-3]. Traditional chromatographic methods areexpensive and results need long turnaround times, making themincompatible with rapid on-site decision making. ELISA methodshave been shown to meet or exceed the performance of gaschromatography—they offer rapid low-cost analysis, therebyincreasing the frequency of sampling and enhancing data quality.Automated ELISA workstations allow the full benefit of these kitsto be realized. Sample preparation, reagent pipetting, incubation,and photometric evaluation can be performed without userintervention. Reliability is increased through the elimination ofoperator error, better accuracy and precision, and often higher speed.Much larger batch sizes are possible and these systems can providesample tracking with report generation for documentationrequirements. In this paper the manual procedures and ELISAmethods are compared and some critical aspects of automating theseELISA kits are discussed.

[学科分类] 分析化学