Erythrocyte and Reticulocyte Indices on the LH 750 as Potential Markers of Functional Iron Deficiency

[摘要] Reticulocyte hemoglobin content (CHr) and percentage of hypochromic cells(%Hypo) are restricted to the Siemens analysers. The aims of the study were to investigate the correlation of Red cells size factor(RSf) and low Hemoglobin density (LHD%), reported by Beckman-Coulteranalysers, with CHr and %Hypo in the assessment of iron status in the presence ofinflammation. 381 samples were run on both LH 750 (Beckman-Coulter) and Advia 2120 (Siemens)analysers. Correlation between parameters were calculated and the diagnosticperformance of the new parameters was assessed.Results. Correlation between RSf and CHr,r=0.85. ROC curve analysis for RSf in the diagnosis of iron restricted erythropoiesis definedas CHr<28pg: AUC 0.983; Cutoff 91.1%; Sensitivity 98.8%; Specificity 89.6% Correlation between LHD% and %Hypo,r=0.869. ROC curve analysis for LHD% in the diagnosis of iron deficiency defined by%Hypo>5%: AUC 0.954; Cut off 6.0%; Sensitivity 96.6%; Specificity 83.2%Conclusions. RSf and LHD% could be reliable parameters for the study of ironmetabolism status.

[学科分类] 血液学