Gene Expression ofIGF1,IGF1R, andIGFBP3in Epiretinal Membranes of Patients with Proliferative Diabetic Retinopathy: Preliminary Study

[摘要] The molecular mechanism formation of secondary epiretinal membranes (ERMs) after proliferative diabetic retinopathy (PDR) or primary idiopathic ERMs is still poorly understood. Therefore, the present study focused on the assessment ofIGF1,IGF1R, andIGFBP3mRNA levels in ERMs and PBMCs from patients with PDR. The examined group comprised 6 patients with secondary ERMs after PDR and the control group consisted of 11 patients with idiopathic ERMs. Quantification ofIGF1,IGF1R, andIGFBP3mRNAs was performed by real-time QRT-PCR technique. In ERMs,IGF1andIGF1RmRNA levels were significantly higher in patients with diabetes compared to control subjects. In PBMCs, there were no statistically significant differences ofIGF1,IGF1R, andIGFBP3expression between diabetic and nondiabetic patients. In conclusion, our study indicatedIGF1andIGF1Rdifferential expression in ERMs, but not in PBMCs, of diabetic and nondiabetic patients, suggesting that these factors can be involved in the pathogenesis or progression of proliferative vitreoretinal disorders. This trial is registered withNCT00841334.

[学科分类] 生理学与病理学