A study of the Interaction Between Cetirizine and Plasma Membrane ofEosinophils, Neutrophils, Platelets and Lymphocytes using A fluorescence Technique

[摘要] The effect of cetirizine on plasma membrane fluidity andheterogeneity of human eosinophils, neutrophils, platelets andlymphocytes was investigated using a fluorescence technique.Membrane fluidity and heterogeneity were studied by measuring thesteady-state fluorescence anisotropy and fluorescence decay of 1-(4-trimethylammonium-phenyl)-6-phenyl-1, 3, 5-hexatriene (TMA-DPH)incorporated in the membrane. The results demonstrate thatcetirizine (1 μg/ml) induced a significant increase in theHpid order in the exterior part of the membrane and a decrease inmembrane heterogeneity in eosinophils, neutrophils and platelets.Moreover, cetirizine blocked the PAF induced changes in membranefluidity in these cells. Cetirizine did not influence significantlythe plasma membrane of lymphocytes. These data may partially explainthe effect ofcetirizine on inflammatory cell activities.

[学科分类] 生理学与病理学