Serum Concentration and Chemotactic Activity of E-selectin (CD62E) in Inflammatory Bowel Disease

[摘要] E-selectin (CD62E) is an endothelial specific glycoprotein belongingto the selectin family of adhesion molecules. Because a highexpression of this molecule at intestinal mucosal surfaces ininflammatory bowel disease (IBD) has been described earlier, the aimwas to assess serum levels of E-selectin (sE-selectin) and tocorrelate it to disease activity, and further to evaluate itschemotactic properties at physiological concentrations. Levels ofsEselectin were measured by a sandwich ELISA technique in 31 IBDpatients together with 15 healthy volunteers. In ulcerative colitisthe median value was 0.46 nM (0.16–0.75), in Crohn’s disease0.47 nM (0.22–1.24), and in healthy controls 0.34 nM(0.22–0.83). No statistically significant differences insE-selectin were revealed between these groups (p>0.05). The in vitro chemotactic capabilities of E-selectin (in theconcentration range of 0.10–31.4 nM) were assessed using theleading front technique. A significantly increased migratoryresponse was found at concentrations of 1.00 (p<0.05) and 3.14 nM (p<0.02). It is concluded that sE-selectin incontrast to sICAM-1 does not act as a sensitive indicator of localimmune activation in IBD. However, E-selectin may be important forrecruitment and accumulation of neutrophilic granulocytes and otherphagocytes involved in the inflammatory process seen in IBD. Futureinvestigations are encouraged in order to reveal itsinvivoeffects.

[学科分类] 生理学与病理学