In vitroRelease of Eosinophil Proteins in Allergic and Atopic Dermatitis Patients

[摘要] To investigate whether eosinophils are stimulatedinvivoor have acquired an increased susceptibility to stimulifrom the coagulation cascade, the release of eosinophil proteins wascompared for three groups of donors with different levels of serumIgE. (1) with atopic dermatitis (s-IgE > 5000 IU/ml,n= 11);(2) with inhalant allergy (200 < s-IgE < 2 000 IU/ml,n= 10); and (3) non-allergic (s- IgE < 100 IU/ml,n= 10). Thelevels of eosinophil cationic protein and eosinophil protein X (ECP,EPX) were determined in serum (clotting time = 2.0 h) and plasma.Serum and plasma ECP in normal donors demonstrated largeintra-personal variations (C.V. 50–80%), but serum-ECP(mean 8.1 ng/ml) was clearly distinguishable from plasma ECP(mean 1.0 ng/ml) by a factor of 8 (range: 5.6–11.6). TheECP released during clotting was markedly increased in the atopicdermatitis group (serum:plasma ratio 13.5,p<0.003) compared with the other groups (6.7 and 5.6). EPX, having a higher plasmalevel, demonstrated a less pronounced release (serum: plasma ratios2.0, 1.7 and 1.4), with no statistical difference between donorgroups. Considering all donors together the levels of ECP and EPX inplasma and in serum were correlated to the number of eosinophils(coefficients of correlation 0.54-0.58,p<0.002).

[学科分类] 生理学与病理学