Acute Phase Protein Response and Polymorphonuclear LeukocyteCathepsin G Release After Slow Interleukin-1 Stimulation in the Rat

[摘要] In this work we have studied the acute phase protein response anddegranulation of polymorphonuclear leukocytesin vivoin the ratafter a slow interleukin-1β stimulation. A total dose of 1 μg, 2 μg,4 μg and 0 μg (controls with only vehicle) of interleukin-1β wasreleased from osmotic minipumps over a period of 7 days. The pumpswere implanted subcutaneously. A cystic formation was formed aroundthe pumps that contained interleukin-1β whereas no tissue reactionwas seen around pumps containing only vehicle. Besides flbroblaststhe cyst wall contained numerous polymorphonuclear leukocytes whichwere positively stained for cathespin G. α2-macroglobulin,α1-inhtbitor-3, α1-proteinase inhibitor, albumin and C3 weremeasured by electroimmunoassay and all showed plasma concentrationpatterns that were dose-dependent to the amount of interleuktn-1βreleased. Fibrinogen in plasma was elevated in the control group butshowed decreased plasma values with higher doses of interleukin-1βreleased. All animals showed increased plasma levels of cathespin Gbut the lowest levels for cathespin G were seen for the highestinterleukin-1β dose released. It was clearly seen that a slowcontinuous release of interleukin-1βin vivocaused an inflammatoryreaction. Plasma levels for the proteins analysed all showed asimilar pattern, namely an initial increase or decrease of plasmaconcentration followed by a tendency to normalization of plasmavalues. It was concluded that a long-term interleukin-1β releasecould not sustain the acute phase protein response elicited by theinitial interleukin-1β release.

[学科分类] 生理学与病理学