Release of IL-1βvia IL-1β-Converting Enzyme in a Skin Dendritic Cell Line Exposed to 2,4-Dinitrofluorobenzene

[摘要] We used a mouse fetal skin dendritic cell line (FSDC) to study theeffect of the strong allergen 2,4-dinitrofluorobenzene (DNFB) on interleukin (IL)-1βrelease and IL-1βreceptor immunoreactivity. Stimulation with DNFB (30 minutes) increasedIL-1βrelease without changing the mRNA levels of the protein. Furthermore, DNFB increased transiently the interleukin-1β-converting enzyme (ICE) activity, as measured with its fluorogenic substrate Z-Tyr-Val-Ala-Asp-AFC. The ICE inhibitor Z-YVAD-FMK prevented the release of IL-1βevoked by DNFB. Incubation of the cells with DNFB (30 minutes) stronglyincreased IL-1βreceptor immunoreactivity. The rapid effect of DNFB on the release of mature IL-1β, without inducing an increase of IL-1βmRNA in FSDC, suggests a posttranslational modification of pro-IL-1βby ICE activity.

[学科分类] 生理学与病理学