Effects after inhalation of (1→ 3)-β-D-glucan in healthy humans

[摘要] Background and aim:This study was performed to assess the effects of an exposure to a pure (1→3)-β-D-glucan, a cell wall component of fungi, plants and certain bacteria.Methods:Twenty-one healthy subjects inhaled saline or (1→3)-β-D-glucan suspended in saline in a random, double-blind, cross-over design. They were examined before exposure and 24 and 72 h afterwards with spirometry, blood sampling and collection of induced sputum. Differential cell counts and eosinophilic cationic protein (ECP) were determined in blood and sputum, and myeloperoxidase (MPO), tumour necrosis factor-α (TNF-α), and interleukin (IL)-8 and IL-10 were determined in sputum supernatants. TNF-α was determined after cultivation of blood mononuclear cells.Results:In sputum, inhalation of saline caused a significant increase in ECP and TNF-α. (1→3)-β-D-Glucan inhalation caused a further increase in these cytokines, although not statistically significantly different from the increase induced by inhalation of saline alone. In blood, the number of eosinophils was significantly decreased 72 h after the challenge with (1→3)-β-D-glucan. This effect was not found after the inhalation of saline alone. TNF-α production from stimulated blood mononuclear cells was significantly decreased 72 h after the (1→3)-β-D-glucan inhalation as compared with the increase induced by saline inhalation.Conclusions:The results suggest that (1→3)-β-D-glucan causes a different type of response as compared with inflammatory agents such as bacterial endotoxin that cause a neutrophil-dominated inflammatory response.

[学科分类] 生理学与病理学