Systemic Administration of Mesenchymal Stem Cells Increases Neuron Survival after Global Cerebral Ischemia In Vivo (2VO)

[摘要] Although many studies have shown that administration of stem cells after focal cerebral ischemiaimproves brain damage, very little data are available concerning the damage induced by globalcerebral ischemia. The latter causes neuronal death in selectively vulnerable areas, including thehippocampal CA1 region. We tested the hypothesis that intravenous infusion of bone marrowderivedstromal cells (mesenchimal stem cells, MSC) reduce brain damage after transient globalischemia. In adult male Sprague-Dawley rats transient global ischemia was induced using bilateralcommon carotid artery occlusion for 20 min in addition to controlled hypotension. Five days after,the animals were anaesthetized with urethane and the brain was fixed, sectioned and stained withhematoxylin-eosin to investigate histological damage. MSC did not fully protect against ischemicdamage, as the number of viable neurons in this group was lower than in normal (sham-operated)rats. However, in MSC-treated rats the number of viable CA1 pyramidal neurons was significallyhigher than in rats that had been subjected to ischemia but not treated with MSC. We conclude thatintravenous administration of MSC after transient global ischemia reduces hippocampal damage.

[学科分类] 精神健康和精神病学