ACTH4 -10, Substance P, and Dizolcipine (Mk-801) Accelerate Functional Recovery After Hemilabyrinthectomy in Goldfish

[摘要] In this study, we evaluated the goldfish modelof hemilabyrinthectomy for investigating potentialrecovery-promoting drugs. In this lesion model,the unilateral removal of the labyrinth induces apostural imbalance in response to light (DorsalLight Reflex), from which the animals canrecover over time. The behavioral effects of twoneuropeptides were tested–namely, of substanceP and ACTH4-10, both of which are known topromote functional recovery in several otherlesion models. Furthermore, the effect of MK-801, an antagonist of the glutamatergic NMDAreceptorsubtype, was tested because thissubstance has also been shown to exert aneuroprotective effect. After lesion of the rightlabyrinth, the animals(n=12)were treated intraperitoneallydaily either with vehicle(n=12),substance P(n=11), ACTH4-10(n=12), or MK-801(n=12). Another group(n=11), which servedas a non-lesion control, did not receive hemilabyrinthectomyor systemic injections. Thelesion group, treated post-operatively with vehicle,did not recover from the postural deviation overthe 24-d testing period. In contrast, all three testsubstances accelerated the functional recoveryafter unilateral labyrinthectomy. The decrease ofthe dorsal light reflex persisted even aftercessation of drug treatment after 20d. Theresults indicate that using the dorsal light reflexin the model of hemilabyrinthectomy in goldfishprovides a useful approach to studying theability of potential new neurotrophic or neuroprotectivedrugs to promote functional recovery.

[学科分类] 精神健康和精神病学