Identification and Characterization of Two Novel RNA Editing Sites ingrin1bTranscripts of EmbryonicDanio rerio

[摘要] Discovering RNA editing sites in model organisms provides an insight into their adaptations in addition to finding potential sites for the regulation of neural activity and the basis of integrated models of metazoan editing with a variety of applications, including potential clinical treatments of neural dysregulation. The zebrafish,Danio rerio, is an important vertebrate model system. We focused on thegrin1bgene of zebrafish due to its important function in the nervous tissue as a glutamate receptor. Using a comparative sequence-based approach, we located possible RNA editing events within thegrin1btranscript. Surprisingly, sequence analysis also revealed a new editing site which was not predicted by the comparative approach. We here report the discovery of two novel RNA editing events ingrin1btranscripts of embryonic zebrafish. The frequency of these editing events and their locations within thegrin1btranscript are also described.

[学科分类] 精神健康和精神病学