Responses of Metabolites in Soybean Shoot Apices to Changing Atmospheric Carbon Dioxide Concentrations

[摘要] Soybean seedlings were grown in controlled environment chambers with CO2partial pressures of 38 (ambient) and 72 (elevated) Pa. Five or six shoot apices were harvested from individual 21- to 24-day-old plants. Metabolites were analyzed by gas chromatography and, out of 21 compounds, only sucrose and fructose increased in response to CO2enrichment. One unidentified metabolite, Unk-21.03 decreased up to 80% in soybean apices in response to elevated CO2. Levels of Unk-21.03 decreased progressively when atmospheric CO2partial pressures were increased from 26 to 100 Pa. Reciprocal transfer experiments showed that Unk-21.03, and sucrose in soybean apices were altered slowly over several days to changes in atmospheric CO2partial pressures. The mass spectrum of Unk-21.03 indicated that this compound likely contained both an amino and carboxyl group and was structurally related to serine and aspartate. Our findings suggested that CO2enrichment altered a small number of specific metabolites in soybean apices. This could be an important step in understanding how plant growth and development are affected by carbon dioxide enrichment.

[学科分类] 农艺学与作物科学