Increased expression of Zinc finger protein 267 in non-alcoholic fatty liver disease

[摘要] Isolation of total cellular RNA from cultured cells and tissue and reverse transcription were performed as described previously [27]. Quantitative real-time PCR was performed with primers specific for ZNF267 (forward: 5'- ATG GGA GCT GTG ATC TTG AGA; reverse: 5'-GCA ATG ATG AAT GAG TAA AGA CC), employing LightCycler technology (Roche, Mannheim, Germany) [28].

[学科分类] 生理学与病理学