Intermittent Compressive Stress Enhanced Insulin-Like Growth Factor-1 Expression in Human Periodontal Ligament Cells

[摘要] Mechanical force was shown to promote IGF-1 expression in periodontal ligament bothin vitroandin vivo. Though the mechanism of this effect has not yet been proved, here we investigated the molecular mechanism of intermittent mechanical stress onIGF-1expression. In addition, the role of hypoxia on the intermittent compressive stress onIGF-1expression was also examined. In this study, human periodontal ligament cells (HPDLs) were stimulated with intermittent mechanical stress for 24 hours.IGF-1expression was examined by real-time polymerase chain reaction. Chemical inhibitors were used to determine molecular mechanisms of these effects. For hypoxic mimic condition, the CoCl2supplementation was employed. The results showed that intermittent mechanical stress dramatically increasedIGF-1expression at 24 h. The pretreatment with TGF-βreceptor I or TGF-β1 antibody could inhibit the intermittent mechanical stress-inducedIGF-1expression. Moreover, the upregulation of TGF-β1 proteins was detected in intermittent mechanical stress treated group. Correspondingly, theIGF-1expression was upregulated upon beingtreated with recombinant human TGF-β1. Further, the hypoxic mimic condition attenuated the intermittent mechanical stress and rhTGF-β1-inducedIGF-1expression. In summary, this study suggests intermittent mechanical stress-inducedIGF-1expression in HPDLs through TGF-β1 and this phenomenon could be inhibited in hypoxic mimic condition.

[学科分类] 分子生物学，细胞生物学和基因