The Bidirectional Transfer and Fetal Vascular Pressure ChangesDue to the Presence of125I-Labeled Inhibin A in theex-vivoHuman Placental Model

[摘要] Objective:The purpose of this study was to investigate the transport of inhibin A and to determine its effectson fetal vascular pressure at elevated levels in the human placenta usingI125-labeled synthetic glycoprotein.Methods: Synthetic inhibinAwas prepared and was shown to be consistent with the natural form by high-pressureliquid chromatography (HPLC) and molecular weight determination by gas-chromatography mass spectrometry.The standardized NaI125process yieldedI125-labeled inhibin A with a radioactivity of106cpm/μg. This compoundwas placed in the human placenta in maternal–fetal and fetal–maternal studies using antipyrine andC14-labeledinulin as controls to determine the bidirectional transfer of the compound.Results: Maternal–fetal and fetal–maternal clearance indices were 0.045± 0.003 and 0, respectively. In eightplacentas there was no evidence of vascular pressure changes due to the presence of up to 5000 pg of inhibin A.Conclusions: There is minimal maternal–fetal transfer and no detectable fetal–maternal transfer in normotensiveand pregnancy-induced hypertensive placentas. In addition, there are no pressure changes in the fetal vascularsystem due to the clinically significant levels of inhibin A.

[学科分类] 妇产科学