Vaginal Microbiota of Healthy Pregnant Mexican Women is Constituted by Four Lactobacillus Species andSeveral Vaginosis-Associated Bacteria

[摘要] Objective. To identify the microbiota communities in the vaginal tracts of healthy Mexican women across the pregnancy.Methods. Vaginal swabs were obtained during the prenatal visit of women from all trimesters (n=64) of healthy pregnant women of Mexico City. DNA was isolated from each sample, and PCR-DGGE and sequencing of 16S rRNA gene fragments were used to identify the bacterial communities.Results. 21 different microorganisms were identified in the vaginal samples.Lactobacillusgenus was present in 98% of women studied. Four lactobacilli species were identified in vaginal samples.L. acidophiluswas the predominant (78%) followed byL. iners(54%),L. gasseri(20%), andL. delbrueckii(6%). 17 different microorganisms related to bacterial vaginosis conditions were identified.Ureaplasma urealyticumwas the predominant (21%) followed by BVAB1 (17%) andGemella bergeriae(7.8%).Conclusions.Lactobacillusgenus predominates in the vaginal samples of Mexican pregnant women associated with different microorganisms related to bacterial vaginosis conditions.

[学科分类] 妇产科学