Persistence of Human Papillomavirus DNA inCervical Lesions After Treatment With DiathermicLarge Loop Excision

[摘要] Objective:The aim of this study was to identify human papillomavirus (HPV) in cervical intraepithelialneoplasia (CIN) lesions and to evaluate the persistence of viral DNA after diathermic largeloop excision (DLLE) treatment.Study Design:Biopsies from 36 patients with low- and high-grade CIN lesions were studiedbefore and after DLLE treatment looking for HPV sequences. DNA was extracted to perform aradioactive polymerase chain reaction (PCR) using GP 5,6 generic primers. PCR products wereanalyzed by the single-stranded conformational polymorphism (SSCP) which is a simultaneousdetection and typing method. Dot-blot hybridization with generic and type-specific biotinylatedoligonucleotide probes was applied in some cases.Results:HPV DNA was found in all pretreatment samples, and the viral type was identified in80% of them, HPV 16 being the most prevalent. The viral type coincided with that detected in thefirst biopsy in all except one case. Seventy five percent of the patients (27 cases) were negative forCIN at follow up, but 50%of them remained HPV DNA positive.Conclusion:DLLE treatment was effective in removing the CIN lesion but not the HPV. Thisfact points out the need to asses the presence of HPV in DNA during the follow-up, since viralpersistence has been considered a high risk factor for recurrence and/or malignant transformation.

[学科分类] 妇产科学