Benzoyl Peroxide Formulated Polycarbophil/Carbopol 934P Hydrogel with Selective Antimicrobial Activity, Potentially Beneficial for Treatment and Prevention of Bacterial Vaginosis

[摘要] The human vagina is colonized by a variety of indigenous microflora; in healthy individuals the predominant bacterial genus isLactobacilluswhile those with bacterial vaginosis (BV) carry a variety of anaerobic representatives of the phylumActinobacteria. In this study, we evaluated the antimicrobial activity of benzoyl peroxide (BPO) encapsulated in a hydrogel againstGardnerella vaginalis, one of the causative agents of BV, as well as indicating its safety for healthy human lactobacilli. Herein, it is shown that in well diffusion assaysG. vaginalisis inhibited at 0.01% hydrogel-encapsulated BPO and that the testedLactobacillusspp. can tolerate concentrations of BPO up to 2.5%. In direct contact assays (cells grown in a liquid culture containing hydrogel with 1% BPO or BPO particles), we demonstrated that hydrogels loaded with 1% BPO caused 6-log reduction ofG. vaginalis. Conversely, three of the testedLactobacillusspp. were not inhibited whileL. acidophilusgrowth was slightly delayed. The rheological properties of the hydrogel formulation were probed using oscillation frequency sweep, oscillation shear stress sweep, and shear rate sweep. This shows the gel to be suitable for vaginal application and that the encapsulation of BPO did not alter rheological properties.

[学科分类] 妇产科学