Impact of Anaerobic Growth Conditions on Toxic Shock Syndrome Toxin-I Production byStaphylococcus aureus

[摘要] Objective:The impact of anaerobic growth conditions on theStaphylococcus aureustoxic shock syndrometoxin-1 (TSST-1) production was studied.Methods:Ten strains ofS. aureusderived from patients with toxic shock syndrome (TSS), 10isolates ofS. aureus, and documented TSST-1-producing strains recovered from patients with eitherstaphylococcal septicemia or staphylococcal nongenital abscesses were grown under aerobic andanaerobic conditions. The bacterial growth was measured using optical density (OD) determinationsat 520 nm. The toxin production was assayed using the TS-RPLA latex agglutination test.Results:Both TSS and non-TSS strains ofS. aureusgrown under aerobic and anaerobic conditionsexhibited comparable OD patterns of growth, and the levels of toxin production remained constantduring the logarithmic phase. Toxin titers developed during the logarithmic growth phase andpeaked after 24 h of incubation. When stationary-phase isolates grown initially under aerobicconditions were subjected to strict anaerobic conditions, subsequent toxin titers, compared withisolates grown in the continued presence of oxygen, were depressed 2-fold, peaking at a later time.Conclusions:TSST-1 production is diminished under continued anaerobic conditions.

[学科分类] 妇产科学