Comparison of Abbott LCxChlamydia trachomatisAssay With Gen-Probe PACE2 and Culture

[摘要] In this study the LCx assay (a nucleic acid amplification assay) forChlamydia trachomatisinendocervical samples was compared with the Gen-Probe PACE2 assay (a nucleic acid probe assay)for endocervical samples, and with endocervical culture. In addition, the efficacy of the LCx assaywas determined for midstream clean-catch urine samples because it is often necessary to obtainsuch a sample for routine urine culture and it is simpler to collect only a single sample without alsocollecting a first-void urine for LCx. Endocervical specimens from 205 patients were tested forC.trachomatisvia LCx and PACE2. Of these patients, 203 were tested by culture. Midstream cleancatchurine samples from 75 of these patients were tested by LCx. The sensitivities and specificitiesfor these assays, after discrepant analysis, were 100 and 98.9% for LCx of endocervical samples,52.4 and 100% for PACE2; and 71.4 and 100% for culture. The sensitivity/specificity of LCx formidstream clean-catch urines was 66.7/98.5%. The apparent prevalence ofC. trachomatisin ourpopulation was 10.2%. These data indicate that among the methods tested, LCx of endocervicalsamples had the highest sensitivity forC. trachomatisin this population. The senstivity of the urineLCx assay using midstream clean-catch collected urines was considerably less than that reportedin other studies that used first-void urines but was higher than that of PACE2. Infect. Dis. Obstet.Gynecol. 8:112–115, 2000.

[学科分类] 妇产科学