Adhesion Development and the Expression of Endothelial Nitric Oxide Synthase

[摘要] Objective:This study was conducted to determine whether nitric oxide (NO), a potent vasodilator and inhibitor of thrombus formation, is involved in the formation and maintenance of adhesions.Methods:Skin, subcutaneous tissues, peritoneum and adhesions were collected from surgical patients and total RNA was isolated. Quantitative reverse transcription polymerase chain reaction (QRT-PCR) was performed toquantitate endothelial nitric oxide synthase (eNOS) and β-actin mRNA levels.Results:eNOS mRNA levels for skin, subcutaneous tissue, peritoneum and adhesions were ≤ 3.12 ×10-4,≤ 3.12 ×10-4, 6.24 ×10-4and 2.5 ×10-3attomoles/μl, respectively. β-actin mRNA levels for all tissues werebetween 1.25 ×10-1and 6.25 ×10-2attomoles/μl.Conclusion:eNOS mRNA can be identified in tissue adhesions, and may therefore play a role in adhesion formation and maintenance.

[学科分类] 妇产科学