Effect of Metronidazole on the Growth of Vaginal Lactobacilliin vitro

[摘要] Objective:To determine whether metronidazole has an adverse effect on the growth ofLactobacillus.Methods:Hydrogen peroxide- and bacteriocin-producing strains ofLactobacilluswere used as test strains.Concentrations of metronidazole used ranged from 128 to 7000 μg/ml. Susceptibility to metronidazole was conductedby the broth microdilution method recommended by the National Committee for Clinical Laboratory Standards.Results:Growth ofLactobacilluswas partially inhibited at concentrations between 1000 and 4000 μg/ml(p= 0.014). Concentrations ≥ 5000 μg/ml completely inhibited growth ofLactobacillus. Concentrations between128 and 256 μg/ml stimulated growth ofLactobacillus(p= 0.025 and 0.005, respectively). Concentrations ofmetronidazole between 64 and 128 μg/ml or ≥ 512 μg/ml did not have an inhibitory or a stimulatory effect on the growth ofLactobacilluscompared to the control.Conclusions:High concentration of metronidazole, i.e. between 1000 and 4000 μg/ml, partially inhibited thegrowth ofLactobacillus. Concentrations ≥ 5000 μg/ml completely suppressed the growth ofLactobacillus.Concentrations between ≥ 128 and ≤ 256 μg/ml stimulated the growth ofLactobacillus. Further investigation todetermine the ideal concentration of metronidazole is needed in order to use the antimicrobial agent effectively inthe treatment of bacterial vaginosis.

[学科分类] 妇产科学