Efecto del Diclofenaco en la formación de osteoclastos inducidos por estrés mecánico

[摘要] Theprostaglandinsestimulateboneresorption.Somestudiesshowthateffectsofprostaglandinsarecontradictory.NSAIDindometacin,aspirinandrefecoxibhavebeenusedforinhibittheosteoclastpopulation.Thegoalofthisworkwasevaluatetheeffectofdiclofenacintheosteoclast´spopulationinratsunderorthodonticalstress.ThirtyWistarmalesratsof250+/-25gofweightwasused.AuniformstandardizedUspring,madeof0.012-inchnickel-titaniumwirewassetineachanimalmouthbetweentherightandleftupperfirstmolar,theyweredividedinto2groupsof15rats,oneofthemwereinyectedwithdiclofenacindosisof2mg/kgeach12hoursTheanimalswerekilledat24,48and72hoursaftertheplacementoftheappliances.Fivemoreratswereusedascontrolwithnodrugandnomechanicalstress.Themaxillaewasremovedandfixedinformaldehyde48hours,descalcifiedinEDTA(pH7.4)andthenprocessedbyhistologicroutinetechnic,andincludedinParafine.Serialsectionsof6?mwerecutandstainedwithhematoxylinandeosin,Results:theNSAIDsdiclofenacinalldosesinhibitedtheformationofosteoclasts.Incontrolgroup,osteoclastsincreasegraduallyat24,48and72hours.Thegroupofwitnesstowhichnothingwasappliedtherewerenoosteoclastsobserved.Inthewitnessgroupsumittedtomechanicalstressthenumberofosteoclstswasgraduallyincreasedat24hours6.41(±.8238)osteoclasts,at48hours9.63(±.8264)osteoclasts,at72hours14.4(±.8039)osteoclast.

[学科分类] 口腔科学