Effect of Gambir Catechin Isolate (Uncaria Gambir Roxb.) Against Rat Triacylglycerol Level (Rattus novergicus)

[摘要] Catechin isolate is an active substance contained in gambir consisting of epicatechin compounds, epicycin-3-galates, epigallocatechin-3-errors and epigallocatechines which have the potential effect to reduce triacylglycerol levels. This study aims to determine the effect of catechin isolates on rat triacylglycerol levels induced by a high fat diet. We use catechin isolates which is an active compound from gambir extract. This study was conducted on 25 rats divided into 5 groups, namely, negative control group (K-), positive control (K +), and 3 treatment groups (P1, P2, P3) given a high-fat diet of cow's brain for 14 days. The treatment group was then given catechin isolates with a dose: 10 mg, 20 mg, and 40 mg/kg body weight/day for 14 days. Triacylglycerol levels were examined using Glycerylphosphate Oxidase (GPO) Method. Data analysis was performed using One way Annova and Post Hoc Tukey HSD. The results showed a decrease in triacylglycerol levels after being given by catechin isolates. Triacylglycerol levels in positive control mice (K +) 147.8 ± 8.5 mg/dL were higher when compared to the negative control group and the treatment group, ie 104.6 ± 15.3 mg/dL, 101.4 ± 15.7 mg/dL, 106.4 ± 17.6 mg/dL, and 110 ± 3.2 mg/dL. There were significant differences in the P1, P2, and P3 groups with the positive control group p = 0.014 (p

[发布机构] Biochemistry Department, Faculty of Medicine, Universitas Andalas, Jl.Perintis Kemerdekaan No 94, Padang; 25127, Indonesia^1

[效力级别] 化学 [学科分类] 生物科学（综合）

[关键词] Active compounds;Active substance;catechin isolate;Gambir;Negative control;Positive control group;Potential effects;Triacylglycerols