Antigenic Protein Profile of Streptococcus mutans Biofilm For Developing of Dental Caries and Periodontal Disease Risk Biomarker

[摘要] Streptococcus mutans is a virulent and biofilm forming bacteria causing dental caries. Dental caries lead to several diseases, including mediastinitis, sepsis, facial cellulitis, osteomyelitis, endocarditis and pneumonia. Alternative effort to suppress dental caries prefalency is early diagnostic of dental caries risk using protein biomarker. The aim of the research is to determine the antigenic protein profile of S. mutans biofilm as biomarker of dental caries and periodontal disease risk. S. mutans biofilm was formed on glass slide submerging in BHIBS media for 24 hours. Crude proteins of biofilm were obtained by lysing it using ultrasonication 7 × 30s, 40 Hz. Protein profiles of S. mutans biofilm was done by using SDS-PAGE with separation gel of 12%. Antigenic analysis of the proteins biofilm were done by Western blot method using sIgA contained in the dental caries free saliva, compared with dental caries saliva as control. Protein profile of S. mutans biofilm consist of seventeen bands of MW 181, 176, 172, 154, 105, 90, 70, 58, 52, 40, 29, 22, 19, 16, 14, and 13 kDa, while the antigenic rection towards sIGA were the protein with MW of 105, 52, and 29 kDa.

[发布机构] Department of Microbiology, Faculty of Sains and Technology, Universitas Airlangga, Surabaya, Indonesia^1;Department of Microbiology, Faculty of Dentistry, Universitas Airlangga, Surabaya, Indonesia^2;Department of Chemistry, Faculty of Sains and Technology, Universitas Airlangga, Surabaya, Indonesia^3

[效力级别] 化学 [学科分类] 生物科学（综合）

[关键词] Crude proteins;Periodontal disease;Protein profiles;S. mutans biofilms;Streptococcus mutans;Streptococcus mutans biofilms;Ultra-sonication;Western blot method